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    Structured Review

    Bio-Rad sds page
    Sds Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+protean+tgx+precast+sds+page+gel/4%E2%80%9320%25+Mini-PROTEAN+TGX+Precast+Gel/pm42826596-93-25-31
    Average 96 stars, based on 1446 article reviews
    sds page - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Protease-activatable scaffold proteins as versatile molecular hubs in synthetic signaling networks
    Article Snippet: .. SDS‐PAGE analysis of purified scaffold proteins and split NanoLuc fusion proteins on a 4‐20% Mini‐ PROTEAN TGX Precast SDS‐PAGE gel (Bio‐Rad). ..

    Article Title: Purification and serological detection of maize yellow mosaic virus.
    Article Snippet: few aphid species, with the primary vector being the corn leaf aphid Rhopalosiphum maidis [8, 18, 27].. MaYMV was first reported in China in sweet corn in 2013 [5, 33] and was subsequently detected in North and South America [3, 8, 13], sub-Saharan Africa [1, 9, 16, 19, 21, 32, 36, 38], and other countries in Asia [15, 17].. MaYMV was even detected in samples from South Africa dating back to 2009 [36], suggesting that the virus has been widespread for some time but has evaded detection.

    SDS Page:

    Article Title: Aromatic cage-directed azide-methyllysine photochemistry for profiling non-histone interacting partners of the MeCP2 methyl-CpG binding domain
    Article Snippet: .. The eluted proteins were separated on a 4-15% Mini-PROTEAN TGX precast SDS-PAGE gel (Bio-Rad, #4561085) and transferred onto a 0.45 μm PVDF membrane at a constant voltage of 80V for 1.5 hr. at 4°C. ..

    Article Title: Purification and serological detection of maize yellow mosaic virus.
    Article Snippet: few aphid species, with the primary vector being the corn leaf aphid Rhopalosiphum maidis [8, 18, 27].. MaYMV was first reported in China in sweet corn in 2013 [5, 33] and was subsequently detected in North and South America [3, 8, 13], sub-Saharan Africa [1, 9, 16, 19, 21, 32, 36, 38], and other countries in Asia [15, 17].. MaYMV was even detected in samples from South Africa dating back to 2009 [36], suggesting that the virus has been widespread for some time but has evaded detection.

    Article Title: UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytes
    Article Snippet: Protein concentration was determined with a Pierce TM Bradford Plus Protein Assay Kit and BSA standard curve (Thermo Fisher Scientific, #23236). .. Sample mastermixes consisting of 15 μg protein extract, 1x dithiothreitol (DTT) (Cell Signaling Technology, #14265), and 1x Blue Loading Buffer (Cell Signaling Technology, #56036) were incubated at 95 °C for 5 min before loading into a 4-20% Mini-PROTEAN TGX precast SDS-PAGE gel (BioRad, #4561096). .. Blue prestained protein marker (11–250 kDa, Cell Signaling Technology, #59329) was also loaded (3.5 μL).

    Article Title: Activation of human FPR2 with AT-RvD1 Resolves Acute Sialadenitis in Vivo
    Article Snippet: .. Equal amounts of total protein (15 μg per well) were loaded onto a 12% Mini-PROTEAN TGX precast SDS-PAGE gel (Bio-Rad) and separated at 100 V for 70 minutes using Tris/Glycine/SDS running buffer. .. Proteins were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad).

    Article Title: UVB-Induced Genotoxic Stress Activates the DNA Damage Response and Innate Immune Pathways in Sea Urchin Coelomocytes
    Article Snippet: Protein concentration was determined with a Pierce Bradford Plus Protein Assay Kit and BSA standard curve (Thermo Fisher Scientific, #23236). .. Sample mastermixes consisting of 15 μg protein extract, 1x dithiothreitol (DTT) (Cell Signaling Technology, #14265), and 1x Blue Loading Buffer (Cell Signaling Technology, #56036) were incubated at 95°C for 5 min before loading into a 4-20% Mini-PROTEAN TGX precast SDS-PAGE gel (BioRad, #4561096). .. Blue prestained protein marker (11 – 250 kDa, Cell Signaling Technology, #59329) was also loaded (3.5 μL).

    Article Title: Activation of Human FPR2 with AT-RvD1 Resolves Acute Sialadenitis in Vivo.
    Article Snippet: .. Equal amounts of total protein (15 μg per well) were loaded onto a 12% Mini-PROTEAN TGX precast SDS-PAGE gel (Bio-Rad) and separated at 100 V for 70 min using Tris/Glycine/SDS running buffer. ..

    Membrane:

    Article Title: Aromatic cage-directed azide-methyllysine photochemistry for profiling non-histone interacting partners of the MeCP2 methyl-CpG binding domain
    Article Snippet: .. The eluted proteins were separated on a 4-15% Mini-PROTEAN TGX precast SDS-PAGE gel (Bio-Rad, #4561085) and transferred onto a 0.45 μm PVDF membrane at a constant voltage of 80V for 1.5 hr. at 4°C. ..

    other:

    Article Title: Novel Approaches to Label the Surface of S. aureus with DBCO for Click Chemistry-Mediated Deposition of Sensitive Cargo.
    Article Snippet: The strain-promoted alkyne−azide cycloaddition (SPAAC) reaction can be used to modify the surface of bacteria for a variety of applications including drug delivery, biosensing, and imaging.. This is usually accomplished by first installing a small azide group within the peptidoglycan and then delivering exogenous cargo (e.g., a protein or nanoparticle) modified with a cyclooctyne group, such as dibenzocyclooctyne (DBCO), for in situ conjugation.. However, DBCO is comparatively bulky and hydrophobic, increasing the propensity of some payloads to aggregate.

    Virus:

    Article Title: Purification and serological detection of maize yellow mosaic virus.
    Article Snippet: few aphid species, with the primary vector being the corn leaf aphid Rhopalosiphum maidis [8, 18, 27].. MaYMV was first reported in China in sweet corn in 2013 [5, 33] and was subsequently detected in North and South America [3, 8, 13], sub-Saharan Africa [1, 9, 16, 19, 21, 32, 36, 38], and other countries in Asia [15, 17].. MaYMV was even detected in samples from South Africa dating back to 2009 [36], suggesting that the virus has been widespread for some time but has evaded detection.

    Incubation:

    Article Title: Purification and serological detection of maize yellow mosaic virus.
    Article Snippet: few aphid species, with the primary vector being the corn leaf aphid Rhopalosiphum maidis [8, 18, 27].. MaYMV was first reported in China in sweet corn in 2013 [5, 33] and was subsequently detected in North and South America [3, 8, 13], sub-Saharan Africa [1, 9, 16, 19, 21, 32, 36, 38], and other countries in Asia [15, 17].. MaYMV was even detected in samples from South Africa dating back to 2009 [36], suggesting that the virus has been widespread for some time but has evaded detection.

    Article Title: UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytes
    Article Snippet: Protein concentration was determined with a Pierce TM Bradford Plus Protein Assay Kit and BSA standard curve (Thermo Fisher Scientific, #23236). .. Sample mastermixes consisting of 15 μg protein extract, 1x dithiothreitol (DTT) (Cell Signaling Technology, #14265), and 1x Blue Loading Buffer (Cell Signaling Technology, #56036) were incubated at 95 °C for 5 min before loading into a 4-20% Mini-PROTEAN TGX precast SDS-PAGE gel (BioRad, #4561096). .. Blue prestained protein marker (11–250 kDa, Cell Signaling Technology, #59329) was also loaded (3.5 μL).

    Article Title: UVB-Induced Genotoxic Stress Activates the DNA Damage Response and Innate Immune Pathways in Sea Urchin Coelomocytes
    Article Snippet: Protein concentration was determined with a Pierce Bradford Plus Protein Assay Kit and BSA standard curve (Thermo Fisher Scientific, #23236). .. Sample mastermixes consisting of 15 μg protein extract, 1x dithiothreitol (DTT) (Cell Signaling Technology, #14265), and 1x Blue Loading Buffer (Cell Signaling Technology, #56036) were incubated at 95°C for 5 min before loading into a 4-20% Mini-PROTEAN TGX precast SDS-PAGE gel (BioRad, #4561096). .. Blue prestained protein marker (11 – 250 kDa, Cell Signaling Technology, #59329) was also loaded (3.5 μL).

    Nucleic Acid Electrophoresis:

    Article Title: Purification and serological detection of maize yellow mosaic virus.
    Article Snippet: few aphid species, with the primary vector being the corn leaf aphid Rhopalosiphum maidis [8, 18, 27].. MaYMV was first reported in China in sweet corn in 2013 [5, 33] and was subsequently detected in North and South America [3, 8, 13], sub-Saharan Africa [1, 9, 16, 19, 21, 32, 36, 38], and other countries in Asia [15, 17].. MaYMV was even detected in samples from South Africa dating back to 2009 [36], suggesting that the virus has been widespread for some time but has evaded detection.



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    (a) Genomic arrangement of the MCR activation operon in M. acetivorans with scale bar depicting 1 kilobase pair. (b) Structure of the putative MCR activation complex (PDB: 9H1L) with MCR in orange, component A2 (with relevant domains highlighted) in blue, and the other methanogenesis marker proteins in gray. NBD refers to N ucleotide B inding D omain and ZBM refers to Z inc B inding M otif. (c) Log 2 transformed FPKM ( F ragments P er K ilobase of transcript per M illion mapped reads) of the MCR activation operon and the MCR operon in M. acetivorans grown in high-salt (HS) minimal medium supplemented with trimethylamine (TMA) at 37 °C. (d) Genotype of an M. acetivorans strain expressing a second copy of component A2 in trans under the control of a tetracycline inducible promotor. (e) Anti-FLAG immunoblot showing the inducible production of component A2 upon the addition of 100 µg/mL tetracycline (tet) to the growth medium in crude and soluble cell lysates with 13.9 µg total protein loaded into each lane. (f) Anti-FLAG immunoblot of aerobic affinity-purification of A2 with a Streptactin resin. The lanes represent the following: (1) ladder, (2) cell lysate, (3) flow-through, (4) first wash, (5) second wash, (6) first elution, (7) second elution, and (8) third elution. <t>(g)</t> <t>SDS-PAGE</t> gel showing anaerobic purification of full-length TAP-tagged component A2 (64 kDa with tag). (h) Anaerobic ATPase assay of component A2 alone (blue), MCR alone (purple), and component A2 combined with MCR (orange). Each reaction contained 500 µg/mL of each protein indicated with 200 µM ATP, 10 mM MgCl 2 , 20 mM HEPES, 300 mM NaCl, and 1% glycerol. Reactions were incubated at 37 °C. Inorganic phosphate production was measured at 0, 15, 30, and 60 minutes using the malachite green reagent. (I) Aerobic ATPase assay of component A2 alone (blue), MCR alone (purple), and component A2 combined with MCR (orange). Proteins were purified anaerobically then removed from the anaerobic chamber and reactions were set up on the bench top. The same assay conditions as (c) were used, but time points were taken at 0, 30, and 60 minutes. Error bars represent the standard deviation of three technical replicates for each reaction.
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    Image Search Results


    (a) Genomic arrangement of the MCR activation operon in M. acetivorans with scale bar depicting 1 kilobase pair. (b) Structure of the putative MCR activation complex (PDB: 9H1L) with MCR in orange, component A2 (with relevant domains highlighted) in blue, and the other methanogenesis marker proteins in gray. NBD refers to N ucleotide B inding D omain and ZBM refers to Z inc B inding M otif. (c) Log 2 transformed FPKM ( F ragments P er K ilobase of transcript per M illion mapped reads) of the MCR activation operon and the MCR operon in M. acetivorans grown in high-salt (HS) minimal medium supplemented with trimethylamine (TMA) at 37 °C. (d) Genotype of an M. acetivorans strain expressing a second copy of component A2 in trans under the control of a tetracycline inducible promotor. (e) Anti-FLAG immunoblot showing the inducible production of component A2 upon the addition of 100 µg/mL tetracycline (tet) to the growth medium in crude and soluble cell lysates with 13.9 µg total protein loaded into each lane. (f) Anti-FLAG immunoblot of aerobic affinity-purification of A2 with a Streptactin resin. The lanes represent the following: (1) ladder, (2) cell lysate, (3) flow-through, (4) first wash, (5) second wash, (6) first elution, (7) second elution, and (8) third elution. (g) SDS-PAGE gel showing anaerobic purification of full-length TAP-tagged component A2 (64 kDa with tag). (h) Anaerobic ATPase assay of component A2 alone (blue), MCR alone (purple), and component A2 combined with MCR (orange). Each reaction contained 500 µg/mL of each protein indicated with 200 µM ATP, 10 mM MgCl 2 , 20 mM HEPES, 300 mM NaCl, and 1% glycerol. Reactions were incubated at 37 °C. Inorganic phosphate production was measured at 0, 15, 30, and 60 minutes using the malachite green reagent. (I) Aerobic ATPase assay of component A2 alone (blue), MCR alone (purple), and component A2 combined with MCR (orange). Proteins were purified anaerobically then removed from the anaerobic chamber and reactions were set up on the bench top. The same assay conditions as (c) were used, but time points were taken at 0, 30, and 60 minutes. Error bars represent the standard deviation of three technical replicates for each reaction.

    Journal: bioRxiv

    Article Title: Component A2 is a redox-sensitive archaeal ATPase activated by methyl-coenzyme M reductase

    doi: 10.64898/2026.03.18.712670

    Figure Lengend Snippet: (a) Genomic arrangement of the MCR activation operon in M. acetivorans with scale bar depicting 1 kilobase pair. (b) Structure of the putative MCR activation complex (PDB: 9H1L) with MCR in orange, component A2 (with relevant domains highlighted) in blue, and the other methanogenesis marker proteins in gray. NBD refers to N ucleotide B inding D omain and ZBM refers to Z inc B inding M otif. (c) Log 2 transformed FPKM ( F ragments P er K ilobase of transcript per M illion mapped reads) of the MCR activation operon and the MCR operon in M. acetivorans grown in high-salt (HS) minimal medium supplemented with trimethylamine (TMA) at 37 °C. (d) Genotype of an M. acetivorans strain expressing a second copy of component A2 in trans under the control of a tetracycline inducible promotor. (e) Anti-FLAG immunoblot showing the inducible production of component A2 upon the addition of 100 µg/mL tetracycline (tet) to the growth medium in crude and soluble cell lysates with 13.9 µg total protein loaded into each lane. (f) Anti-FLAG immunoblot of aerobic affinity-purification of A2 with a Streptactin resin. The lanes represent the following: (1) ladder, (2) cell lysate, (3) flow-through, (4) first wash, (5) second wash, (6) first elution, (7) second elution, and (8) third elution. (g) SDS-PAGE gel showing anaerobic purification of full-length TAP-tagged component A2 (64 kDa with tag). (h) Anaerobic ATPase assay of component A2 alone (blue), MCR alone (purple), and component A2 combined with MCR (orange). Each reaction contained 500 µg/mL of each protein indicated with 200 µM ATP, 10 mM MgCl 2 , 20 mM HEPES, 300 mM NaCl, and 1% glycerol. Reactions were incubated at 37 °C. Inorganic phosphate production was measured at 0, 15, 30, and 60 minutes using the malachite green reagent. (I) Aerobic ATPase assay of component A2 alone (blue), MCR alone (purple), and component A2 combined with MCR (orange). Proteins were purified anaerobically then removed from the anaerobic chamber and reactions were set up on the bench top. The same assay conditions as (c) were used, but time points were taken at 0, 30, and 60 minutes. Error bars represent the standard deviation of three technical replicates for each reaction.

    Article Snippet: Samples were then loaded into 12% precast Tris-Glycine denaturing SDS-PAGE gels (Mini-PROTEAN TGX, Bio-Rad, Hercules, CA, USA).

    Techniques: Activation Assay, Marker, Transformation Assay, Expressing, Control, Western Blot, Affinity Purification, SDS Page, Purification, ATPase Assay, Incubation, Standard Deviation